The Association of the Polymorphic Marker of +795 G>A in the Adiponectin Receptor 2 Gene with Biopsy-confirmed Metabolic Dysfunction-associated Steatotic Liver Disease

Kosar Babaeian Roshani; Zahra Ourang; Mitra Rostami; Atefeh Dehghanitafti; Mobina Hosseini; Touraj Mahmoudi; Ronak Soltanirazlighi; Aidin Mahban; Radmehr Shafiee; Helia Sadat Kaboli; Gholamreza Rezamand; Asadollah Asadi; Reza Dabiri; Seidamir Pasha Tabaeian

doi:10.15403/jgld-6326

Authors

Kosar Babaeian Roshani Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Zahra Ourang Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Mitra Rostami Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Atefeh Dehghanitafti Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Mobina Hosseini Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Touraj Mahmoudi Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Ronak Soltanirazlighi Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Aidin Mahban Department of Business Management, Science and Research Branch, Islamic Azad University, Tehran, Iran
Radmehr Shafiee Sterile Services Department (CSSD), Practice Plus Group Hospital, Emersons Green, Bristol, United Kingdom
Helia Sadat Kaboli Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Gholamreza Rezamand Department of Internal Medicine, School of Medicine, Iran University of Medical Sciences, Tehran; Colorectal Research Center, Iran University of Medical Sciences, Tehran, Iran
Asadollah Asadi Department of Biology, Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran
Reza Dabiri Internal Medicine Department, Semnan University of Medical Sciences, Semnan, Iran
Seidamir Pasha Tabaeian Department of Internal Medicine, School of Medicine, Iran University of Medical Sciences, Tehran; Colorectal Research Center, Iran University of Medical Sciences, Tehran, Iran

DOI:

https://doi.org/10.15403/jgld-6326

Keywords:

Adiponectin, ADIPOR2, MASLD, rs16928751, variant

Abstract

Background and Aims: Metabolic dysfunction-associated steatotic liver disease (MASLD) with the prevalence of around 25% is the most prevailing cause of chronic liver disease. In this study, we aimed to investigate the association of the rs16928751 or +795 G>A variant in adiponectin receptor 2 gene (ADIPOR2) with MASLD.

Methods: Genomic DNA was isolated from the whole blood of 130 patients with biopsy-confirmed MASLD, and 130 controls according to the phenol-chloroform extraction and ethanol precipitation approach. Then the polymorphic marker of +795 G>A was genotyped by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).

Results: The +795 G>A variant met the Hardy-Weinberg equilibrium (p > 0.05) in both control and patient groups; hence, the samples had good population representativeness. The genotype count of ADIPOR2 gene +795 G>A differed significantly between these two groups. The +795 G>A „AA” genotype in comparison to the „GG” was more frequent in the patients with MASLD (p=0. 037; OR=2.24, 95%CI: 1.20–6.47).

Conclusions: To our knowledge, this study is the first one that found a significant association between the +795 G>A variant of the ADIPOR2 gene and biopsy-confirmed MASLD; nonetheless, it needs to be corroborated by further research in different populations.